Neurobiology is the study of cells of the nervous system and the organization of these cells into functional circuits that process information and mediate behavior. In this course we will explore molecular and cellular aspects of neuronal physiology, their organization into higher systems and approach methodologies used to analyze CNS function at different levels. Such techniques will include recent progress in whole brain imaging, advances in fluorescence microscopy and optogenetics, the basics of single-cell sequencing and the use of cellular, organoids or animal models in neuroscience. We will also discuss deviations from neuronal physiology such as during aging or after onset of CNS related pathologies, including neurodegenerative diseases and approaches to cell reprogramming and regeneration in order to recover cellular function.At the end of this course, students will have a broader understanding on techniques used to study neuronal function at a molecular, cellular and systemic level and will have the basics insights to infer which tools are more appropriate depending on the application.