This course focuses on principles and applications in cell engineering. Class lectures include an overview of molecular biology fundamentals, protein/ligand binding, receptor/ligand trafficking, cell-cell interactions, cell-matrix interactions, and cell adhesion and migration at both theoretical and experimental levels. Lectures will cover the effects of physical (e.g. shear stress, strain), chemical (e.g. cytokines, growth factors) and electrical stimuli on cell function, emphasizing topics on gene regulation and signal transduction processes. Furthermore, topics in metabolic engineering, enzyme evolution, polymeric biomaterials, and drug and gene delivery will be discussed. This course meets with EN.580.441 but includes additional requirements designed for the core curriculum of the RIE (Regnerative and Immune Engineering) track of the BME masters program. The course is also appropriate for Cell & Tissue Engineering Ph.D. students and may be taken by advanced undergraduate students upon permission of the instructor. Prerequisites: Graduate standing with background in cell biology and biochemistry or EN.580.221 or AS20.305 and AS.020.306 (or equivalent) and AS.030.205 or permission of the instructor.